THE METABOLISM OF L-THREONINE AND GLYCINE BY Escherichia coli
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چکیده
منابع مشابه
Glycine Synthesis and Metabolism in Escherichia Coli.
Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine and glycine. Serine synthesis from 3-phosphoglycerate was observed in the same ...
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Amino-acid producers have traditionally been developed by repeated random mutagenesis owing to the difficulty in rationally engineering the complex and highly regulated metabolic network. Here, we report the development of the genetically defined L-threonine overproducing Escherichia coli strain by systems metabolic engineering. Feedback inhibitions of aspartokinase I and III (encoded by thrA a...
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A rapid method for purifying L-threonine dehydrogenase from Escherichia coli K-12 cells, grown on a medium containing L_-threonine as carbon source, has been developed. The procedure consists of three fractionation steps of which the last is adsorption and elution of the enzyme from a column of Blue dextran-Sepharose. Homogeneous and stable samples of the dehydrogenase are obtained. L-Threonine...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1957
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.43.2.195